Angiogenesis, the process by which new blood vessels form from existing ones, is an important part of the tissue repair process. Adequate blood supply to tissues is essential for delivering oxygen and nutrients during the repair process, and research into factors that support angiogenesis has been an active area of regenerative medicine study. Amniotic tissue contains naturally occurring factors that the scientific literature associates with angiogenic activity.
This post takes a closer look at two of those factors and what the published literature says about their roles.
VEGF — Vascular Endothelial Growth Factor
VEGF is one of the most studied angiogenic factors in the scientific literature. It is a signaling protein reported to play a central role in the formation and regulation of new blood vessels. VEGF is naturally present in amniotic tissue and has been a subject of significant research interest in the context of tissue biology and regenerative medicine.1,2
As a component of AlphaFlo®, VEGF is present as part of the naturally occurring biological profile of canine amniotic tissue. The manufacturing process is designed to preserve the native tissue and retain its naturally occurring proteins, including VEGF.
EGF — Epidermal Growth Factor
EGF is a signaling protein that plays a role in regulating cell growth, proliferation, and survival. It is involved in the signaling pathways associated with tissue repair and has been studied extensively in the context of wound biology and regenerative medicine research.1,2
Like VEGF, EGF is a naturally occurring component of amniotic tissue and is present in AlphaFlo® as part of its native biological composition.
Why the Biological Profile Matters
The combination of anti-inflammatory, anti-fibrotic, immunomodulatory, and angiogenic factors naturally present in canine amniotic tissue is what makes AlphaFlo® a biologically active product. Unlike freeze-dried or lyophilized products, AlphaFlo® is manufactured and cryopreserved to help maintain these factors in their native state, preserving the biological consistency of the tissue across batches.
AlphaFlo® does not claim specific clinical outcomes. VEGF and EGF are naturally occurring components of canine amniotic tissue and are present in AlphaFlo®. Clinical decisions regarding the use of AlphaFlo® are made at the discretion of the attending veterinarian.
See how veterinarians across the country are incorporating AlphaFlo® into their care plans: https://alphaflocanine.com/category/case-study/
Key Takeaway
AlphaFlo® contains angiogenic factors including VEGF and EGF that support vascular development and cellular signaling.
VEGF is a signaling protein reported in the scientific literature to play a central role in the formation and regulation of new blood vessels, while EGF is involved in the signaling pathways associated with cell growth and tissue repair.
Both factors are naturally present in canine amniotic tissue and are preserved in AlphaFlo® through the manufacturing process, which is designed to maintain their biological characteristics in a native state.
The angiogenic profile of AlphaFlo® completes a broader biological picture that also includes anti-inflammatory, anti-fibrotic, and immunomodulatory factors naturally found in canine amniotic tissue.
Angiogenic Factors in AlphaFlo®
| Factor | Full Name | Primary Function | Why It Matters |
| VEGF | Vascular Endothelial Growth Factor | Supports blood vessel formation | Promotes vascular development and nutrient delivery to tissues. |
| EGF | Epidermal Growth Factor | Supports cellular growth and signaling | Contributes to normal tissue maintenance and cellular activity. |
References
- McQuilling JP, Vines JB, Kimmerling KA, Mowry KC. Proteomic Comparison of Amnion and Chorion and Evaluation of the Effects of Processing on Placental Membranes. Wounds. 2017;29(6):E36-E40.
- Avilla-Royo E, et al. Comprehensive quantitative characterization of the human term amnion proteome. Matrix Biol Plus. 2021;12:100084.